Pentaphosphorylated Magic Spot Nucleotides: Chemoenzymatic Synthesis and Disassembly-Based Sensing

Key Points

• The central aim is to enhance the detection and accessibility of magic spot nucleotides in bacterial systems.
• Developed a scalable chemoenzymatic approach for synthesizing MSNs.
• Investigated the disassembly-based mechanisms for sensing MSNs.
• Applied biochemical assays to validate the selective detection of MSNs.
• Successfully synthesized pentaphosphorylated magic spot nucleotides.
• Achieved selective detection of (p)ppGpp and (p)ppApp in bacterial systems.
• Demonstrated improved chemical accessibility for further study of stress signaling pathways.