Scaling-Up Vertical-Wheel Bioreactors Based on Cell Aggregate Exposure to Shear Stress and Energy Dissipation Rate

Key Points

• To understand how shear stress and energy dissipation rates affect the performance of vertical-wheel bioreactors with cell aggregates.
• Investigated the effects of energy dissipation rates on cell aggregates in vertical-wheel bioreactors.
• Examined shear stress implications on hPSC aggregation and expansion.
• Compared trajectory-based cell aggregate exposures with volume-averaged energy dissipation rates.
• Shear stress did not significantly influence hPSC aggregation or proliferation.
• Trajectory-based exposures provided a better scale-up basis for bioreactors than volume-averaged methods.