Computer-Aided Quantitative Image Analysis of Multiple Human Epidermal Growth Factor Receptor 2 Immunohistochemistry Assays in Breast Carcinoma

Key Points

• The aim is to evaluate the effectiveness of a quantitative image analysis method for HER2 scoring in breast carcinoma.
• Utilized a computer-aided quantitative image analysis (QIA) method.
• Assessed multiple immunohistochemistry (IHC) assays.
• Compared HER2 classical overexpression and HER2-low levels.
• The QIA method was accurate for HER2 scoring across different IHC assays.
• It was agnostic to the assay type, ensuring consistent results.
• The method can reliably distinguish between HER2-positive and HER2-low breast carcinomas.

Abstract