Determination of human skin permeation is necessary in drug development for topical treatments, the cosmetics sector, and occupational hazard evaluation applications. When compared to the in-vitro assay experiments based on Franz cells for the determination of human skin permeation, the estimation methods based on surrogate chromatographic models are less time-consuming and can be easily automated. In this work, we evaluated surrogate chromatographic alternative organic mobile modifiers, acetone, tetrahydrofuran, and 2-propanol, for the estimation of human skin permeation of small neutral organic compounds using surrogate chromatographic systems. Solvent systems comprising 70% (v/v) acetone and 70% (v/v) 1:1 tetrahydrofuran: 2-propanol on a Kinetex C18 stationary phase demonstrated the best results for the construction of surrogate chromatographic models with prediction errors of 0.180-0.231 and 0.214-0.254 log units, respectively. Proposed surrogate chromatographic models could be used as a rapid screening tool to estimate human skin permeation of small neutral organic compounds.
Atapattu et al. (Wed,) studied this question.