Construction of saturated Tn-Seq libraries of Brucella abortus S19 for transposon insertion and effective density analysis across stress conditions

Key Points

• The aim is to investigate the functional relevance of Brucella abortus genes in various stress conditions.
• Constructed high-density transposon sequencing libraries in B. abortus S19 strain
• Analyzed transposon insertion frequencies under different stress conditions
• Evaluated effective densities of gene insertions
• Identified specific genes associated with resilience to acidic, oxidative, and antimicrobial stresses
• Provided quantitative data on transposon insertion frequencies
• Demonstrated significant variations in insertion rates across tested stress conditions