Molecular profiling of gene-edited cells reveals shared drug-resistance mechanisms

Key Points

• The research aims to identify common molecular mechanisms that contribute to drug resistance in gene-edited skin cancer cells.
• Developed a platform named PerturbFate to track gene expression and chromatin changes.
• Applied PerturbFate to skin-cancer cells with various genetic perturbations.
• Analyzed the dedifferentiated state of cells post-interventions.
• Different genetic perturbations lead to a shared dedifferentiated state.
• Many drug-resistance mechanisms operate through common molecular pathways.