Biocatalytic Site-Selective Desaturation: A Tool for Late-Stage Functionalization of Complex Molecules

Key Points

• The aim is to develop a biocatalytic approach for site-selective dehydrogenation of complex molecules.
• Utilized engineered enzymes from ERED for selective dehydrogenation reactions.
• Employed directed evolution to optimize enzyme selectivity.
• Tested multiple cyclic ketones and complex terpenoid and steroid structures.
• Achieved selective dehydrogenation at specific sites within complex substrates.
• Demonstrated successful modification of various cyclic ketones.
• Increased efficiency and specificity in functionalizing terpenoids and steroids.