ABSTRACT A rapid and reliable electrophoretic method was developed for the chiral separation of DL‐phenylalanine (DL‐PHE) enantiomers using S‐β‐cyclodextrin (S‐β‐CD) as a chiral selector. Key parameters influencing enantioresolution including chiral selector type and concentration, buffer pH, applied voltage, and temperature were systematically optimized. Under optimal conditions, baseline separation of the DL‐PHE racemate was achieved within 6 min with a resolution factor (Rs) of 4.75. The analysis was performed in a fused‐silica capillary using a 25 mM phosphate buffer (pH 2.5) containing 1.125 mM S‐β‐CD as the background electrolyte. Electrophoretic separation was carried out in normal polarity mode at 25°C with an applied voltage of 22 kV and hydrostatic injection for 7 s. The proposed method was validated and met the criteria for selectivity, linearity, precision, and accuracy/recovery. The validated procedure was successfully applied to the determination of DL‐PHE and L‐PHE in commercial food supplements, demonstrating its suitability for routine chiral quality control and analytical applications.
Elbashir et al. (Wed,) studied this question.