ABSTRACT Environmental DNA (eDNA) has become a transformative tool for biodiversity monitoring and conservation across Southeast Asia, one of the world's most critical biodiversity hotspots. Despite its potential, several factors hinder the adoption and scalability of eDNA tools in the region. In this perspective paper for the special issue “Understanding the Current Use of Environmental DNA in Southeast Asia: Promoting Accessibility through Networking and Capacity Building,” we synthesize our insights as eDNA researchers and end‐users across the region to identify key challenges and highlight opportunities for enhanced regional networking, capacity building, and collaboration. Key challenges we identified include limited resources, the lack of standardized protocols, particularly in the context of metabarcoding, and the lack of training in eDNA data analysis. Hurdles such as outdated infrastructure, expensive reagents and equipment costs, logistical barriers, and limited bioinformatics expertise also persist. Furthermore, initiatives to advance and standardize eDNA methods, protocols, and technologies are often led by the Global North, which may neglect to account for Southeast Asia's distinct ecological landscape and resource challenges. Based on these insights, our key recommendations include the development of regional eDNA laboratories, the creation of adaptable standards that reflect the region's wide range of biodiversity and environmental conditions, investment in eDNA training, and building local bioinformatics expertise. We also highlight major themes such as the need for adaptive standards, regional collaboration, and potentially repurposing COVID‐19 surveillance infrastructure for eDNA studies, demonstrating how existing molecular platforms can be leveraged for biodiversity monitoring. By aligning regional efforts with global standards while addressing local needs, researchers in Southeast Asia can maximize the potential of eDNA for biodiversity conservation and ecological research. Future eDNA research in Southeast Asia will depend on continued innovation, collaboration, and the development of methodologies tailored to the region's unique biodiversity.
Lopez et al. (Sun,) studied this question.