Abstract This study aimed to compare the performance of polyethylene glycol (PEG) precipitation, and Nanotrap ® Microbiome magnetic particle capture workflows for recovering novel fecal marker, pBI143 from 12 wastewater samples collected across six treatment plants in Maryland, USA. Quantitative PCR (qPCR) was used to quantify marker abundance. The Nanotrap workflow yielded significantly higher concentration of pBI143 compared to PEG precipitation workflow ( p 0.05) were observed, whereas the concentration of PMMoV was significantly lower than that of the three fecal markers ( p < 0.05). Based on the concentration alone, pBI143, ToBRFV, and crAssphage were found to be a better alternative to PMMoV as an endogenous fecal marker.
Ali et al. (Fri,) studied this question.