What question did this study set out to answer?

This study examines the association between the CARD8 gene polymorphism rs2043211 and dental caries susceptibility.

April 22, 2026Current Research in Dental Sciences0 citationsOpen Access

Linkage Analysis of Polymorphism of CARD8 Gene (SNP: rs2043211) in Relation to Dental Caries: A Case-Control Study

HHHashim Mueen Hussein SSSura Sabah AMAthraa Ali Mahmood

Key Points

This study examines the association between the CARD8 gene polymorphism rs2043211 and dental caries susceptibility.
Case-control study with participants aged 20-30 years
Divided into healthy (non-carious) and decayed groups
Sequenced CARD8 gene SNP rs2043211 from peripheral blood samples.
No significant differences in genotype distribution between healthy and decayed participants (P>0.3)
Odds Ratio for AT genotype indicates a potential association with decay (OR=1.83, P=0.3)
Allelic variations did not show significant differences, suggesting further research needed.

Abstract

Objective: Teeth decay disease is a huge health problem and some reports have inferred caspase recruitment domain family member 8 (CARD8), inflammasome sensor involvement in the disease. Several single nucleotide polymorphisms (SNPs) have been detected in the CARD8 gene. The reference SNP 2043211 (rs2043211) variant is situated in its exon region, leading to the dental caries. The study aimed to determine the impact of the CARD8 (rs2043211SNPs) on the susceptibility of caries presence in patients.Methods: The case-control study included 38 participants aged 20-30 years divided into two groups; the first group (17) was healthy with non-carious teeth and the other group (21) was with active decayed teeth. The clinical examination was done for caries detection then the peripheral blood was taken from all included participants of both groups. The CARD8 gene SNP rs2043211 was amplified and sequenced.Results: Hardy-Weinberg Equilibrium (HWE) analysis of CARDS8 at SNP rs2043211 showed a non-significant (NS) difference in both studied groups. The Adenine Thymine (AT) genotype among the decayed participants was 13 SNPs while in healthy participants 8 SNPs with a non-significant (NS) difference (P=.3). Nevertheless, the odds Ratio (OR) of the AT genotype was 1.83 (0.49-6.7, P=.3) and for genotype TT, it was 1.3 (0.3-5.6, P=.7). It was observed that SNP rs2043211 was associated with tooth decay. In addition, OR for the A allele is 0.6 (0.024-1.5) and 1.65 (0.6-4.15) for the T allele with P=.2 for both alleles.Conclusion: Many factors can play an important role in the initiation and progression of the caries directly or indirectly. The allelic variations in the CARD8 gene are to have been associated with the development of decayed teeth among patients.Keywords: CARD8 gene polymorphism, dental caries, sequencing, teeth decay

Demander à l'IA

Bookmark

View Full Paper