Analysis of Cholesterol Induced Mechanical Modulation of Cell Membrane by Nanopipette-Generated Fluid Flow

Key Points

• This research aims to investigate how cholesterol affects the mechanical properties of living cell membranes using a novel assay.
• Utilized nanopipette-generated fluid flow to create membrane oscillations.
• Employed label-free detection based on ionic current fluctuations to assess alterations.
• Focused on live cell membranes for real-time observations.
• Detected significant mechanical alterations in cell membranes due to cholesterol presence.
• Demonstrated the effectiveness of nanopipette-generated flow for membrane analysis.
• Observed oscillation patterns correlating to cholesterol levels.