The gut cells and symbiotic bacteria play a critical role in maintaining gut health and influencing disease development, with metabolic interactions among its constituents warranting further investigation. In this study, we developed a cell-bacteria coculture system on a microfluidic gut chip to simulate the human gut microenvironment and performed multi-omics analyses to elucidate the metabolic crosstalk within the system. The results revealed that the coculture significantly enhances the adhesion and biofilm formation of symbiotic bacteria to gut epithelial cells through activation of the glucose-pyruvate-acetate metabolic cycle. This coculture promotes glucose consumption and acetate secretion while remaining resilient to antibiotics. Moreover, the coculture protected symbiotic bacterial biofilms from antibiotic-induced disruption, thereby enhancing colonization resistance and improving the efficacy of antibiotics against pathogens. Our findings highlight the importance of cell-bacteria interactions in driving the glucose-pyruvate-acetate metabolic cycle, enhancing symbiotic adhesion, and optimizing antibiotic efficacy. • Development of cell-bacteria coculture on a microfluidic gut chip with key attributes of symbiotic adhesion • Gut cell-bacteria coculture enhances symbiotic adhesion and biofilm formation • Enhanced adhesion and biofilm formation are driven by glucose-pyruvate-acetate metabolic cycle • The coculture enhances colonization resistance and antibiotic efficacy against pathogens
Li et al. (Wed,) studied this question.