Optimized Cas‐SF01 gene‐editing toolbox shortens flowering timing in commercial maize inbred JING724

Key Points

• This study aims to evaluate the effectiveness of the optimized Cas-SF01 gene-editing toolbox in shortening flowering time in maize.
• Optimized Cas-SF01-TREX2 configuration was employed for gene editing.
• Focus on maximizing efficiency and achieving high-purity gene mutations.
• Evaluated impact on flowering timing in commercial maize inbred JING724.
• Maize flowered seven days earlier with no loss in yield.
• High-purity gene mutations achieved confirmed effectiveness of the Cas-SF01 toolbox.