Spleen-targeted DDS-based immunization: A novel platform for rapid and highly diversified antibody generation

Key Points

• The aim is to develop a novel immunization method using DDS technology for efficient antibody generation.
• Developed a spleen-targeted immunization method using PEG-modified liposomes.
• Delivered antigens directly to spleen marginal zone B cells.
• Compared antibody responses against model antigens OVA and membrane protein ACE2.
• Achieved superior antibody responses compared to traditional methods.
• Induced high titers of diverse and high-affinity neutralizing antibodies.
• Confirmed effectiveness for membrane proteins including GPCRs.