Yangxinshi Tablet ameliorated post-myocardial infarction heart failure in rats by inhibiting FOXO1/PDK4 signaling, enhancing TCA cycle activity, and elevating ATP production.
Does Yangxinshi Tablet improve cardiac function and energy metabolism in a rat model of post-myocardial infarction heart failure?
Yangxinshi Tablet exerts cardioprotective effects in post-MI heart failure by improving energy metabolism through the inhibition of FOXO1/PDK4 signaling.
Heart failure (HF) is a major contributor to global morbidity and mortality, with myocardial infarction (MI)-induced HF accounting for a substantial proportion of cases. Although Yangxinshi Tablet (YXS) is clinically used, the mechanisms by which it alleviates HF remain unclear. To elucidate the protective mechanisms of YXS in post-MI HF. An MI-induced HF model was established in male Sprague-Dawley rats, and cardiac function, exercise endurance, hemodynamics, serum biochemical indices, and pathological damage were assessed. To investigate the underlying mechanisms, metabolomics, quantitative polymerase chain reaction (qPCR), ribonucleic acid sequencing (RNA-seq), Western blot, immunofluorescence, chromatin-immunoprecipitation (ChIP)-qPCR, and single-cell RNA-seq were employed. The components of YXS were analyzed via molecular docking, and their biological activity was validated in cell-based assays. YXS improved cardiac function and exercise endurance, enhanced hemodynamic parameters, reduced inflammatory cell infiltration, and decreased collagen fiber deposition in vivo. In vitro, YXS regulated mitochondrial energy metabolism and protected against oxygen-glucose deprivation (OGD)-induced cardiomyocyte injury. Notably, YXS ameliorated post-MI HF by inhibiting forkhead box O1 (FOXO1)/pyruvate dehydrogenase kinase 4 (PDK4) signaling, thereby promoting the tricarboxylic acid (TCA) cycle and increasing adenosine 5'-triphosphate (ATP) levels to restore energy metabolism both in vivo and in vitro. Senkyunolide H, apigenin, astragaloside IV, and astragaloside VII were identified as active constituents of YXS using an OGD-induced H9c2 cell injury model. These findings indicate that YXS exerts cardioprotective effects in a rat model of post-MI HF. Mechanistically, YXS inhibits FOXO1/PDK4 signaling, enhances TCA cycle activity, and elevates ATP production to improve cardiac energy metabolism and restore energy homeostasis.
Guo et al. (Fri,) conducted a other in post-myocardial infarction heart failure. Yangxinshi Tablet (YXS) was evaluated on cardiac function, exercise endurance, hemodynamics, serum biochemical indices, and pathological damage. Yangxinshi Tablet ameliorated post-myocardial infarction heart failure in rats by inhibiting FOXO1/PDK4 signaling, enhancing TCA cycle activity, and elevating ATP production.