Aging miRNA Reference Catalog: 84 Candidates with Integrated Hallmark, Centenarian-Signature, Target-Footprint, Tissue-Exposure, SASP Overlay, and 504-Pair IP-Clearance Dossiers Across Six Antisense Modulator Chemistries

Public reference catalog of 84 aging-dysregulated human miRNAs with integrated dossiers covering Lopez-Otin hallmark mapping, centenarian-signature classification across six independent cohort studies, predicted target footprint, GTEx v8 tissue exposure across ten aging-relevant tissues, mouse and rat ortholog mapping, SenMayo senescence-associated secretory phenotype (SASP) overlay statistics, top fifteen HIGH-tier predicted targets, and freedom-to-operate clearance across six antisense modulator chemistries (LNA-2'-MOE-PS gapmer 16 nt, 2'-MOE-PS gapmer 22 nt, mimic 22 nt, GalNAc-2'-MOE-PS gapmer 16 nt, PMO 22 nt, tough-decoy 60 nt) against a 5, 596-patent corpus across 252 assignees. The catalog is published as field infrastructure under CC-BY-4. 0. An academic group, a biotech R every freedom-to-operate verdict is reproducible from the bundled per-pair clearance JSON against the named 5, 596-patent corpus on the snapshot date. Twenty-five candidates pass the canonical LNA-2'-MOE-PS gapmer 16 nt chemistry without any HIGH or MEDIUM patent encumbrance flag against the surveyed corpus and are publishable at canonical antisense specificity without licensing concern: miR-7-5p, miR-9-5p, miR-23a-3p, miR-31-5p, miR-99a-5p, miR-101-3p, miR-124-3p, miR-128-3p, miR-129-5p, miR-138-5p, miR-150-5p, miR-153-3p, miR-184, miR-191-5p, miR-203a-3p, miR-204-5p, miR-219-5p, miR-223-3p, miR-335-5p, miR-365a-3p, miR-369-3p, miR-409-3p, miR-449a-5p, miR-485-5p, miR-494-3p. Eight candidates classify as DIRECTSASP under hypergeometric over-representation of senescence-associated secretory phenotype genes (Saul 2022 SenMayo, 125-gene human signature) among their top-500 strongest predicted binders at p < 0. 05. Six of the eight are also IP-clean at canonical LNA-2'-MOE-PS gapmer 16 nt: miR-130a-3p, miR-130b-3p, miR-148a-3p, miR-148b-3p, miR-152-3p, miR-365a-3p. These six are the natural starting point for a senolytic discovery program targeting the SASP axis directly via miRNA modulation. The two ENCUMBERED DIRECTSASP candidates (miR-19a-3p, miR-19b-3p) clear at design-around chemistries (mimic 22 nt, PMO 22 nt, tough-decoy 60 nt). The hero candidate is miR-130a-3p, the only candidate in the catalog that hits all three independent evidence axes simultaneously: SASP-axis (top-500 predicted binders contain twelve SenMayo signature genes including EREG, IGF1, IL15, TNFRSF1B, MMP13, MMP10, TNF, IGFBP5, with log2 fold-enrichment 1. 917 and hypergeometric p = 8. 3 x 10^-5), centenarian-preserved (preserved-LLI in the German-Kiel PBMC cohort of ElSharawy 2012, Aging Cell, with confidence MEDIUM), and IP-clean canonical (CLEAR at LNA-2'-MOE-PS gapmer 16 nt with HIGH = 0 and MEDIUM = 0 across the 5, 596-patent corpus). The literature-evidence corpus is 559 audited rows after a five-layer pre-launch audit (citation existence via CrossRef on every distinct DOI, numerical consistency across all summary fields, claims-match-data verification on every dossier headline, SASP x centenarian x intervention overlay reconciliation, prior-art pre-flight on the IP-clean SASP-axis subset). 464 rows (83. 0%) are A-grade primary-source verified; the remaining 95 are B-grade primary-traceable review-cited. All twelve Lopez-Otin hallmarks are explicitly covered (h1=6 / h2=9 / h3=9 / h4=15 / h5=8 / h6=28 / h7=89 / h8=353 / h9=85 / h10=46 / h11=136 / h12=2). The bundle additionally ships the 21-row centenarian-preserved-LLI signature (six independent cohorts: Hackl 2010 Vienna PBMC, Smith-Vikos 2016 BLSA serum, Borras 2021 Spanish-Valencia plasma, ElSharawy 2012 German-Kiel PBMC, Olivieri 2012/2013 Italian-Marche endothelial / plasma, Noren Hooten 2010/2013 US-NIA PBMC + serum), a 24-row intervention-responsive table (caloric restriction, rapamycin, NAD+, metformin, exercise), and the full 504-pair clearance grid as per-pair JSON. Each per-candidate dossier carries: family + tier + miRBase v22 ID; Lopez-Otin hallmark coverage from the audited literature evidence; centenarian-signature classification with cohort list, methods, tissue, therapeutic implication, caveat, and confidence; per-chemistry CLEAR / ENCUMBERED clearance verdict with HIGH / MEDIUM / LOW counts (per-pair detail in clearance/) ; recommended cleanest chemistry; predicted target footprint x GTEx v8 expression across the top three tissues by raw repressive footprint and the top three by enrichment; cross-species translation listing mouse and rat orthologs with seed-family coverage; top conserved targets ranked by TargetScan weighted context score; SenMayo SASP overlay with classification (DIRECTSASP / SASPBORDERLINE / NONSASPAXIS), top-500 hypergeometric statistics, and the top SenMayo hits ranked by binding affinity; top fifteen HIGH-tier predicted targets with TargetScan-conserved flag; the full audited literature-evidence rows for the candidate; and the PubMed / DOI reference list. Tiers reflect literature-evidence depth, not therapeutic priority: Tier 1 (multi-hallmark anchors, 3 or more Lopez-Otin hallmarks): 10 candidates including let-7a-5p, miR-29a/b/c-3p, miR-181a/b-5p, miR-34a-5p, miR-21-5p, miR-146a-5p, miR-126-3p. Tier 2 (two-hallmark coverage): 29 candidates. Tier 3 (single-hallmark coverage including paralog clusters and SASP-axis discovery): 45 candidates. The clearance check is freedom-to-operate due diligence on the surveyed corpus snapshot date, not a Section 102 prior-art filing. The catalog does not assert that any patent claim is invalid; it asserts only that the candidate sequence + chemistry, as published in this catalog at the dossier-described specificity, does not read on a HIGH-severity claim of any surveyed patent on the corpus snapshot date. Method-of-treatment, formulation, combination, and dosing claims are not scored. Filings between the corpus snapshot date and any later use should be re-checked. The catalog does not claim therapeutic efficacy; predicted target footprints, tissue exposure, and SASP overlays are computational predictions calibrated against literature, with bench validation and clinical trials remaining the standard for therapeutic assertions. The catalog does not include or expose Coracle Research's underlying computational methodology; the predictor implementation that produces the binding-affinity numbers is held proprietary and is not part of this deposit. Companion research note at https: //www. coracleresearch. com/research/06-aging-mirna-platform/. Deposit roughly 48 MB on Zenodo (382 MB extracted, 603 files).