The non-linear effects of the number of stochastic single-molecule adsorption events on ensemble elution profiles

Key Points

• This research explores how non-linear effects impact adsorption events in chromatography.
• Utilized stochastic model of chromatography to analyze adsorption events.
• Employed single-molecule microscopy for visualization of molecular analytes.
• Examined elution profiles based on varying numbers of adsorption events.
• Identified significant non-linear effects on ensemble elution profiles.
• Demonstrated the impact of single-molecule events on molecular mass transfer.
• Highlighted potential optimizations for chromatographic separation techniques.