Mechanistic Implications of Excited High-Spin States, Spin-Spin Coupling, and Differential 2Fe–2S+ Cluster Temperature Relaxations in the Electron-Bifurcating NfnABC from Thermococcus sibiricus

Key Points

• The aim is to explore the mechanistic implications of high-spin states and coupling in electron bifurcation.
• Analyzed excited high-spin states and spin-spin coupling
• Assessed differential temperature relaxations of [2Fe–2S]+ clusters
• Studied the electron-bifurcating NfnABC enzyme from Thermococcus sibiricus
• Identified key features of excited high-spin states on energy transduction
• Demonstrated comprehensive insights into coupling mechanisms
• Found significant implications for biological energy efficiency in redox reactions.