PrfH accelerates repair of colicin E3 - damaged translating ribosomes by RtcB2

Key Points

• To investigate the role of PrfH in enhancing ribosome repair by RtcB2 on colicin E3-damaged ribosomes.
• Utilized recombinant components to build a ribosome repair system.
• Analyzed repair of colicin E3-rRNAse cleaved 30S subunits and 70S ribosomes by RtcB2 alone.
• Assessed the effect of PrfH on ligation efficiency of damaged ribosomes during translation.
• RtcB2 effectively repaired colicin E3-damaged 30S and 70S ribosomes.
• PrfH significantly increased the efficiency of ribosome repair by RtcB2.
• The study provides insight into the ribosome repair mechanisms facilitating translation.