What question did this study set out to answer?

The research aims to develop an Argonaute-based assay for measuring deaminase activity toward specific substrates.

May 7, 2026Analytical Chemistry0 citations

An Argonaute-Based Assay for Synchronous Quantification of Deaminase Catalytic Activity toward Cytidine and 5-Methylcytidine Substrates

Key Points

The research aims to develop an Argonaute-based assay for measuring deaminase activity toward specific substrates.
Developed an Argonaute-Based Assay for deaminases.
Tested the assay for catalytic activity toward cytidine and 5-Methylcytidine.
Evaluated performance across a pH range of 5.5 to 8.5 in cellular lysates.
Assay provides accurate measurement comparable to glycosylase-based methods.
Demonstrated robustness in varying pH conditions.
Established an efficient platform for functional and kinetic analysis of CDAs.

Abstract

and 4.7 μM, respectively, which align well with those from conventional glycosylase-based assays. ABDD also proved to be robust over a broad pH range of 5.5 to 8.5 and enabled accurate measurement in complex cellular lysates. In summary, the ABDD assay establishes a versatile and efficient platform for the functional and kinetic analysis of CDAs, offering a powerful tool with broad applicability in related research fields.

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Cite This Study

Xu et al. (Mon,) studied this question.

synapsesocial.com/papers/69fbe3ca164b5133a91a3176 https://doi.org/https://doi.org/10.1021/acs.analchem.5c06831

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