Validation of a UHPLC-DAD Method for the Quantification of Glucosinolates in Capsella bursa-pastoris (L.)

Key Points

• To validate a UHPLC-DAD method for accurately measuring glucosinolates in Capsella bursa-pastoris.
• Developed a UHPLC-DAD method for glucosinolate quantification
• Conducted validation experiments for accuracy and precision
• Analyzed glucosinolate content in various samples of Capsella bursa-pastoris.
• The UHPLC-DAD method showed over 95% accuracy in glucosinolate quantification
• Detection limits for glucosinolates were established at ≤0.5 mg/L
• Validation parameters met the acceptable criteria for food quality analysis.