Abstract: Accelerating orthodontic tooth movement, within its acceptable limits, can prevent damage to the teeth and its surrounding structures that are usually caused by prolonged treatment. Recent studies have investigated new methods and techniques that can reduce treatment time. It has been reported that Glutathione can induce osteoclastic differentiation, potentially accelerating tooth movement. Thus, this study aimed to determine the effect of reduced glutathione (GSH) on orthodontic tooth movement in rabbits. This in-vivo study utilized twenty-six (26) 10-12 week old male rabbits that were divided into 3 groups: Control Group A (without treatment), Control Group B (with tooth movement only) and Experimental Group C (with tooth movement and GSH). A nickel-titanium (Ni-Ti) closed coil spring with a 56.7g force was inserted between the maxillary incisors and the first molar to allow tooth movement. Reduced glutathione (GSH) 100 mg was orally administered daily, one week prior to and after the installation of the appliance. After 14 days, the distance of tooth movement was measured. H&E staining was performed to determine osteoclastic activity and observed under a scanning electron microscope at 10X and 40X magnification. Results showed that the mean distance of orthodontic tooth movement (mm) among subjects without GSH was at 0.20mm while for those with GSH was at 0.41mm. The number of osteoclasts also differed between those without GSH which had 1-2 osteoclasts and those with GSH which had 3-4 osteoclasts. The correlation using Spearman Rho’s test regardless of the groups was strongly positive at Spearman’s rank correlation coefficient (rs)= 0.95. Among those without GSH, it was found to be lower but still moderately positive at rs = 0.67. Lastly, those treated with GSH also had a strongly positive correlation at rs =0.84. All of the results were statistically significant at alpha =0.01. It can be concluded that there was an increase in tooth movement in the group that received 100mg GSH compared to the group without GSH. Keywords: orthodontic tooth movement, reduced glutathione, osteoclastic activity. Title: Increased orthodontic tooth movement using 100mg Reduced Glutathione (GSH) on rabbits Author: Patricia Nicole C. Macalintal, Cecilia Angela C. Clemente International Journal of Healthcare Sciences ISSN 2348-5728 (Online) Vol. 14, Issue 1, April 2026 - September 2026 Page No: 64-71 Research Publish Journals Website: www.researchpublish.com Published Date: 27-April-2026 DOI: https://doi.org/10.5281/zenodo.19812909 Paper Download Link (Source) https://www.researchpublish.com/papers/increased-orthodontic-tooth-movement-using-100mg-reduced-glutathione-gsh-on-rabbits
Macalintal et al. (Mon,) studied this question.