Stability Modification of Therapeutic Aptamers: From Biostability Bottlenecks to Nuclease-Resistant Construct Design

Key Points

• The aim is to overcome biostability issues in nucleic acid aptamers to enhance their therapeutic potential.
• Developed nuclease-resistant aptamer constructs through synthetic modifications.
• Evaluated binding affinity and specificity of modified aptamers against target molecules.
• Assessed the biostability of constructs in biological environments.
• Modified aptamers showed significantly improved biostability compared to standard constructs.
• Enhanced binding affinity was observed, with a notable increase in therapeutic target modulation.
• Overall improvements suggest greater potential for clinical applications in drug delivery.

Abstract