A pseudoviral human rhinovirus reference material and RT-dPCR method were developed to enable traceable RNA quantification in SI units, exhibiting stability for 12 months at -80 °C.
The development of a pseudoviral HRV reference material and traceable quantification method enhances the accuracy and comparability of HRV detection across laboratories.
OBJECTIVES: This study developed a pseudoviral human rhinovirus (HRV) reference material using pseudovirus technology and integrating RT-dPCR with HPLC-IDMS to achieve accurate HRV RNA quantification, aiming to standardize HRV detection across labs and platforms. METHODS: gene. RESULTS: copies/μL (k=2). The RM exhibited sufficient homogeneity and stability for 12 months at -80 °C, and its non-infectious nature coupled with its ability to simulate the entire workflow including nucleic acid extraction offers significant advantages. CONCLUSIONS: This study provided essential tools for standardizing HRV detection across different laboratories and platforms. This work enables traceable RNA quantification in SI units and simulates the full analytical process, thereby enhancing the accuracy and comparability of results. Furthermore, this approach serves as a transferable model for developing reference materials and traceable quantification methods for other respiratory pathogens, contributing to advancements in molecular diagnostics and public health.
Li et al. (Wed,) conducted a other in Human rhinovirus (HRV) detection. Pseudoviral HRV reference material and RT-dPCR with HPLC-IDMS was evaluated on HRV RNA quantification accuracy and stability. A pseudoviral human rhinovirus reference material and RT-dPCR method were developed to enable traceable RNA quantification in SI units, exhibiting stability for 12 months at -80 °C.