Abstract Background: Staphylococcus aureus is a significant and widespread human bacterial pathogen that is associated with both hospital- and community-acquired illnesses globally. Objectives: The purpose of the study was to characterize the sequences of the SCCmecA type IV and pvl genes in community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) isolates from various cutaneous infections. Materials and Methods: Intensive care unit burns, private clinics, and Al-Hilla Teaching Hospital provided samples from 100 different skin infections for our study. S. aureus was recognized using normal culture media and microscopic inspection. Utilizing the Vitek-2 susceptibility system, the antibiotic sensitivity test was conducted. Polymerase chain reaction (PCR) screening of the 16sRNA , mecA , SCCmecA type IV, and pvl genes was carried out. Results: Phenotypic detection of MRSA was detected by cefoxitin and oxacillin screening test using the Vitek2 system. The results of this study demonstrated that all of the isolates 20 (100%) were resistant to cefoxitin and oxacillin. Conventional PCR showed that 83.33%, 80%, 50%, 55% of S. aureus were positive for the 16sRNA , mecA , SCCmecA type IV, and pvl genes, respectively. Conclusion: The current study revealed high S. aureus isolates in burns, followed by impetigo, wounds, and boils, respectively. In contrast, there were no bacterial isolates in acne or folliculitis. In addition, the genotypic method is more accurate than the phenotypic one for detecting MRSA and is considered the gold standard method. Previously. This extremely important CA-MRSA, hitherto believed to be exclusive to the hospital setting, is now also being isolated from community settings. The majority of CA-MRSA carry the pvl gene and have the easily transferable tiny mobile SCCmec elements IV.
Al-Hassnawi et al. (Thu,) studied this question.