Establishment of a peptide-based electrochemical immunosensor for detecting antibodies against the GP5 protein from the porcine reproductive and respiratory syndrome virus

Key Points

• To develop a peptide-based electrochemical immunosensor for detecting antibodies against PRRSV in pig serum.
• Utilized electrochemical impedance spectroscopy (EIS) to measure changes in electron transfer resistance.
• Functionalized a gold electrode with synthetic viral peptide for specific antibody binding.
• Tested the sensor with pig serum samples to monitor antibody presence.
• Successfully detected anti-PRRSV antibodies, indicating high sensitivity of the sensor.
• Demonstrated significant changes in electron transfer resistance upon antigen-antibody binding.
• Validation shows promising application potential for disease monitoring in livestock.