Linggui Zhugan Decoction significantly improved cardiac function and pathological morphology in rats with post-myocardial infarction heart failure by modulating F-actin cytoskeletal remodeling.
Does Linggui Zhugan Decoction improve cardiac function and F-actin cytoskeletal organization in a rat model of post-myocardial infarction heart failure and H9C2 cardiomyocytes?
Linggui Zhugan Decoction improves cardiac function and F-actin cytoskeletal stability in preclinical models of post-myocardial infarction heart failure, potentially via RhoA targeting.
BACKGROUND Linggui Zhugan Decoction (LGZGD), a traditional Chinese formula for spleen-strengthening and yang-warming to resolve fluid retention, has consistently shown efficacy in preventing and treating heart failure. However, its underlying biological mechanisms remain incompletely understood. OBJECTIVE This study aimed to investigate the therapeutic efficacy of LGZGD in a rat model of heart failure and in H9C2 cardiomyocytes, as well as to elucidate its effects on F-actin remodeling and the underlying mechanisms. METHODS A rat model of heart failure post-myocardial infarction was established by ligating the left anterior descending coronary artery, followed by treatment with LGZGD for 4 weeks. The chemical constituents of the decoction and drug-containing serum were characterized using ultra-performance liquid chromatography. Network pharmacology analysis identified potential key therapeutic targets of LGZGD for heart failure treatment. An in vitro hypoxia/reoxygenation injury model was constructed in H9C2 cardiomyocytes. Additionally, siRNA-mediated ROCK knockdown was performed to investigate the mechanisms underlying LGZGD-mediated regulation of F-actin remodeling in cardiomyocytes. RESULTS The results showed that LGZGD significantly improved cardiac function and pathological morphology in heart failure rats. Network pharmacology analysis identified RhoA as a key potential target for LGZGD in regulating F-actin in heart failure. Both in vivo and in vitro experiments further confirmed that LGZGD modulates F-actin cytoskeletal remodeling. CONCLUSION The present findings indicate that LGZGD significantly improves cardiac function in rats with heart failure. Furthermore, it enhances the stability of F-actin cytoskeletal organization and function in both heart tissues from rats with heart failure and H9C2 cardiomyocytes.
Cheng et al. (Mon,) conducted a other in Heart failure post-myocardial infarction. Linggui Zhugan Decoction (LGZGD) was evaluated on Cardiac function and pathological morphology. Linggui Zhugan Decoction significantly improved cardiac function and pathological morphology in rats with post-myocardial infarction heart failure by modulating F-actin cytoskeletal remodeling.