In this study, we present a novel method to enhance the resolution of fluorescence optical microscopy based on a fluorescence emission difference configuration. In the first stage, a subtraction technique with a small number of subtraction coefficients is introduced to improve spatial resolution while minimizing the occurrence of negative intensity values, thereby preserving image information consistent with the imaging characteristics of the optical system. Subsequently, an image processing procedure is developed based on the optical transfer properties of the system to further enhance image resolution. Both simulation and experimental results demonstrate that the proposed method not only achieves a significant improvement in image resolution but also effectively reduces the loss of information commonly observed in conventional subtraction‐based approaches.
Vannhu Le (Thu,) studied this question.