Milk Neoallergen in Orange Cheese Rinds?

Key Points

• The research aims to identify potential neoallergens in orange cheese rinds using immunoblot techniques.
• Conducted immunoblot analysis with patient's serum on extracted proteins from cheese rinds.
• Separated 15 g of total proteins using SDS-PAGE and transferred them onto a PVDF membrane.
• Performed Western blotting with a dilution of serum at 1/50 to detect IgE-reactive bands.
• Identified specific IgE-reactive bands indicating the presence of potential neoallergens in cheese.
• Chemiluminescence successfully highlighted these reactive proteins, suggesting allergenic properties.