The efficient delivery and pH-sensitive release of anti-tumor drugs by nanocarriers have been recognized as an appealing strategy to enhance the chemotherapeutic efficacy toward acidic tumors. Here, we present a protocol for preparing magnesium-doxorubicin (Mg-DOX) liposomes as a pH-sensitive drug delivery system with a remote-loading approach. We detail the steps for the construction of MgAc2 liposomes as the precursor, drug encapsulation, and formulation of the final product. We also describe the procedures for characterizing the liposome preparation. For complete details on the use and execution of this protocol, please refer to Yang et al.1
Yu et al. (Tue,) studied this question.