Elastic enhancer network tunes equilibrium thermodynamics of liquid liquid phase separation in super enhancers

denotes the interaction strength between enhancer elements, thereby limiting droplet growth and supporting SE size homeostasis in the nucleus. Model predictions agree quantitatively with experiments and indicate that mechanical constraints regulate the critical concentration and miscibility of SE-associated droplets. Mechanical constraints may potentially regulate local concentration by altering the osmotic pressure difference across the droplet interface, which may in turn modulate transcriptional activity.