The Position of Hydrophobic Residues Impacts Cellular Uptake and Intracellular Localization of Cell Penetrating Peptides

Key Points

• This research aims to understand how the position of hydrophobic residues affects the uptake and localization of cell-penetrating peptides within cells.
• Evaluated various hydrophobic residues in cell-penetrating peptides
• Analyzed cellular uptake using mammalian cell models
• Assessed intracellular localization and endosomal escape
• Hydrophobic residues significantly improved cellular uptake and endosomal escape rates.
• Specific positioning of these residues correlated with enhanced intracellular localization.
• The study underscores the importance of peptide design in optimizing delivery.