The occurrence of the European brown hare (Lepus europaeus Pallas 1778) has declined throughout Europe in recent years. However, it remains economically valuable as an important game species. To date, information on the individual microbiota of the European hare has been limited. The phylum Firmicutes (Bacillota) was dominant, and enterococci belong to this phylum. However, they can carry virulence factor genes. Therefore, this study aimed to address two aspects: the health of hares due to their recent decline, and, as a game animal, the protection of consumers’ health. Based on MALDI-TOF mass spectrometry, five strains were identified as Enterococcus faecium and two as E. faecalis; these findings were confirmed by genotyping using PCR and phenotypic analysis. The average value of lactic acid production was 0.680 ± 0.005 mmol/L. The strains lacked the virulence factor genes esp, agg, and gelE. However, they showed susceptibility to antibiotics and to postbiotic substances, even to 13 of 14 tested. PS/Ent M appears to be the most active PS against tested strains, with inhibitory activity of 25,600 AU/mL. Postbiotic substances represent a new tool for preventing unwanted microbiota in game animals.
Lauková et al. (Thu,) studied this question.