Engineering a Novel Cofactor‐Versatile Short‐Chain Dehydrogenase for Efficient Synthesis of Chiral Alcohols

Key Points

• This research aims to develop a versatile short-chain dehydrogenase for the synthesis of high-value chiral alcohols, focusing on conversion efficiency.
• Utilized glucose as a cosubstrate with CFA as the biocatalyst.
• Measured conversion and enantiomeric excess (e.e.) of chiral alcohol synthesis.
• Evaluated space-time yield (STY) for efficiency.
• Achieved 92.1% conversion of CFA within 6 hours.
• Obtained > 99.9% enantiomeric excess (e.e.) for the product.
• Attained a space-time yield of 368.2 g/(L·d).