This research aims to develop a sensing platform for accurate analysis of 5-hydroxymethylcytosine across the genome.
Utilized a label-free sensing strategy.
Employed a cascade amplification technique without template dependence.
Evaluated the performance using receiver operating characteristic (ROC) curve analysis.
Achieved an area under the ROC curve (AUC) of 0.993, indicating high sensitivity and specificity.
Demonstrated potential applications in tumor liquid biopsies and epigenetic screening.
Abstract
< 0.001), with an area under the receiver operating characteristic curve (AUC) of 0.993, holding potential applications in tumor liquid biopsy and epigenetic marker screening.