Author response of: Comparing DNA extraction methods for successful PacBio HiFi sequencing: a case study of the freshwater mussel Anodonta anatina (Bivalvia: Unionidae). Round#2

Key Points

• This response aims to address the effectiveness of various DNA extraction methods for PacBio HiFi sequencing in Anodonta anatina.
• Comparison of different DNA extraction protocols.
• Evaluation of their impact on sequencing quality.
• Case study focused on freshwater mussel Anodonta anatina.
• Specific extraction methods resulted in higher quality DNA for sequencing.
• Enhanced read accuracy and completeness were noted with optimized protocols.
• Findings support best practices for future genetic studies in mollusks.