What question did this study set out to answer?

This research aims to develop a novel intronic artificial microRNA strategy for plant gene silencing.

May 18, 2026Plant Communications0 citationsOpen Access

CRISPR-Mediated Intronic Knockin of Pre-amiRNA Enables Targeted Gene Silencing

JDJinsong DongInstitute of Crop Sciences KZKe ZhangCenter for Excellence in Molecular Plant Sciences MSMinglei SongSouthern University of Science and Technology

Key Points

This research aims to develop a novel intronic artificial microRNA strategy for plant gene silencing.
Implemented CRISPR/Cas9-mediated knock-in for amiRNA precursor insertion into introns of target genes.
Focused on achieving tissue-specific gene silencing without persistent transgene expression.
Effectively silenced target genes in multiple plant tissues.
Achieved significant reductions in target gene expression, enhancing functional genomic studies.

Abstract

We developed an intronic artificial microRNA (IamiRNA) strategy that combines CRISPR/Cas9-mediated knock-in with endogenous miRNA processing for plant gene silencing. By inserting amiRNA precursors into introns of endogenous genes, this approach enables effective and tissue-specific gene silencing without persistent transgene expression, offering a promising tool for functional genomics and crop improvement.

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Cite This Study

Dong et al. (Fri,) studied this question.

synapsesocial.com/papers/6a0aac2b5ba8ef6d83b6fa53 https://doi.org/https://doi.org/10.1016/j.xplc.2026.101916

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