CRISPR/Cas9 Recombineering with Bacteriophage Recombinases Enables Efficient and Scarless Genome Editing in Cupriavidus necator H16

Key Points

• The research aims to develop an efficient method for genome editing in Cupriavidus necator using CRISPR/Cas9 and bacteriophage recombinases.
• Utilized CRISPR/Cas9 technology for genome editing
• Employed bacteriophage recombinases to facilitate recombineering
• Focused on strain development for metabolic engineering applications.
• Achieved efficient genome editing with high precision
• Demonstrated scarless modifications in the Cupriavidus necator genome
• Showed potential for improved C1 metabolic pathways in industrial applications.