A novel CRISPR/Cas14a-synergized wood-based platform for ultrasensitive detection of aflatoxin B1

Key Points

• The aim is to develop a platform for the ultrasensitive detection of aflatoxin B1 without the need for preamplification.
• Developed a CRISPR/Cas14a-synergized wood-based platform.
• Performed assays to optically identify aflatoxin B1.
• Achieved detection of aflatoxin B1 at 0.67 fmol/L.
• Demonstrated high sensitivity and specificity in assays.