KIBRA impairs DNA damage repair and suppresses osteosarcoma progression by inhibiting the Wnt/β-catenin signaling activity

Key Points

• This research aims to understand how KIBRA affects DNA damage repair and osteosarcoma progression.
• KIBRA overexpression was studied to observe its effects on tumor growth and DNA damage repair.
• Biomarkers γH2AX foci and RAD51 expression levels were measured as indicators of DNA damage and repair efficacy.
• The role of Wnt/β-catenin signaling was assessed using the activator SKL2001 to counter KIBRA effects.
• KIBRA overexpression resulted in smaller tumor sizes and impaired DNA damage repair, shown by increased γH2AX foci and decreased RAD51 levels.
• Activation of the Wnt/β-catenin pathway with SKL2001 reversed KIBRA's inhibitory effects on both osteosarcoma progression and DNA repair.
• KIBRA demonstrates tumor suppressor functions by negatively regulating the Wnt/β-catenin signaling pathway.