Spectroscopy-Assisted Bayesian Optimization for Efficient Refolding of Inclusion Body Proteins

Key Points

• This research aims to enhance the efficiency of refolding recombinant proteins that form insoluble inclusion bodies in Escherichia coli.
• Utilized spectroscopy to monitor protein refolding processes.
• Employed Bayesian optimization to identify optimal refolding conditions.
• Assessed the impact of these methods on protein yield and solubility.
• Achieved significant improvement in protein yield compared to conventional refolding techniques.
• Demonstrated enhanced solubility of refolded proteins, with quantitative metrics provided in detailed analysis.