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Colorectal cancer (CRC) is a malignant tumor of the digestive tract, and previous studies demonstrated that soluble dietary fiber from foxtail millet (FMB-SDF) exhibits significant anti-colorectal cancer effects both in vivo and in vitro. However, the metabolic changes of FMB-SDF in colorectal cancer models remain incompletely understood. This study focused on FMB-SDF. Using AOM/DSS to induce CRC in C57BL/6 J mice, an in vivo colorectal cancer model was established. LC-MS untargeted metabolomics technology was used to detect fecal metabolites in each group of mice to find out the potential biomarkers of CRC, to screen the potential metabolic pathways of FMB-SDF to intervene in CRC, and then to explore the mechanism of action of the anti-cancer effect of FMB-SDF based on the metabolic pathways. This study found that FMB-SDF can regulate some microbial metabolites in the intestine, enhance oxidative stress in colon cancer cell line HCT116, inhibit the secretion of inflammatory factors, and exert anti colorectal cancer effects. From the perspective of sphingolipid metabolism, after treatment with Myriocin, the recovery effect of FMB-SDF on weight loss, colon shortening, and intestinal barrier damage in colon cancer mice decreased, confirming that the anti colon cancer effect of FMB-SDF is closely related to the regulation of sphingolipid metabolic pathway. This provides new insights into FMB-SDF as a functional food for the prevention and treatment of colorectal cancer. • The anti CRC effect of FMB-SDF is closely related to the regulation of sphingolipid metabolism. • FMB-SDF can enhance oxidative stress in colon cancer cells. • FMB-SDF can inhibit the secretion of inflammatory factors. • FMB-SDF can promote cell apoptosis. • FMB-SDF can alleviate the development of CRC by regulating intestinal metabolites.
Yang et al. (Sat,) studied this question.