This study investigated the effects of different straw return practices—no-tillage with straw mulching (SM), shallow tillage with straw incorporation (SS), and deep tillage with straw incorporation (DS)—on the content and structural characteristics of soil water-soluble organic carbon (WSOC) under a maize–soybean rotation in the black soil region in the Northeast of China. Compared with SM, SS and DS increased WSOC content by 39.0% and 28.8% in the 0~20 cm layer (p < 0.05), and by 28.4% and 8.5% in the 20–40 cm layer, respectively. Deep tillage combined with straw return reduced the WSOC/SOC ratio. The DS treatment exhibited the highest levels under maize straw incorporation, while SM treatment showed the highest levels under soybean straw incorporation. Spectral indices in both maize and soybean seasons—including the fluorescence index (FI, ranging from 1.53 to 1.57 in the maize season and from 1.53 to 1.67 in the soybean season), biological index (BIX, ranging from 0.84 to 1.79 in the maize season and from 0.61 to 0.74 in the soybean season), and humification index (HIX, ranging from 0.51 to 0.79 in the maize season and from 0.84 to 0.97 in the soybean season)—collectively indicated that WSOC predominantly consisted of microbially processed organic matter with a low degree of humification. PARAFAC modeling resolved two fluorescent components in maize season: C1 (humic acid-like substances, accounting for 34.8–54.9%) and C2 (Tryptophan-like substance, accounting for 45.1–65.2%), and two components in the soybean season: C1 (humic-like substances, 51.0–53.7%), and C2 (Fulvic acid-like substance 46.3–49.0%). Overall, deep straw return promotes soil humification but increases the structural complexity of WSOC. This systematic investigation provides mechanistic insights into how straw return practices regulate the quantity and quality of labile carbon pools in agricultural ecosystems over time.
Kuang et al. (Tue,) studied this question.