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Cryopreservation has seen limited application in Pyrus communis germplasm due to low post-thaw regeneration rates and strong genotype-dependence. This study aimed to establish a standardized cryopreservation platform encompassing post-thaw regeneration, in vitro rooting, and greenhouse acclimatization, which is applicable across genetically diverse European pear accessions. We implemented optimized cryopreservation and in vitro rooting protocols developed in our laboratory on 11 P. communis accessions with varied genetic backgrounds. All accessions exceeded the Food and Agriculture Organization/International Plant Genetic Resources Institute (FAO/IPGRI) minimum viability threshold of 40%, achieving an average regeneration rate of 75.4%. Most accessions exhibited high in vitro rooting efficiency (≥79.2%), except for one accession (53.3%). Genetic stability was evaluated using inter-simple sequence repeat (ISSR) and amplified fragment length polymorphism (AFLP) markers, with no detectable polymorphisms observed between cryopreserved and non-cryopreserved plants. Phenotypic assessments under greenhouse conditions confirmed overall morphological consistency, with only minor variations observed during the early growth stage. This cryopreservation strategy provides a reliable, reproducible method for long-term conservation and recovery of P. communis germplasm, preserving genetic integrity and offering a scalable foundation for future large-scale propagation and distribution to support breeding and commercial cultivation.
Song et al. (Thu,) studied this question.