In vivo imaging reveals temperature sensitivity of innate immune cell behavior and tissue regeneration in zebrafish

Key Points

• This research investigates how temperature affects the behavior of immune cells and tissue regeneration in zebrafish.
• In vivo imaging was utilized to observe immune cell recruitment across five different temperatures.
• Neutrophil and macrophage responses were measured to determine their association with varying thermal conditions.
• The impact of temperature on tail regeneration was also analyzed.
• Neutrophil recruitment increased steadily with higher temperatures.
• Macrophage recruitment peaked at around 31°C, indicating a narrow optimal thermal window.
• Excessively high temperatures were shown to disrupt immune resolution and the regeneration of tissue.