What question did this study set out to answer?

The aim is to evaluate the antiviral effect of compounds while ensuring cellular toxicity is assessed.

May 29, 2026Open Access

AVITHRAPID - Standard Operating Procedure - Antiviral activity evaluation by qPCR – Virucidal compounds

Key Points

The aim is to evaluate the antiviral effect of compounds while ensuring cellular toxicity is assessed.
Test the highest dose with >90% cell viability for antiviral assays.
Assess viral load in supernatants from infected cell cultures.
Use Vero E6 for SARS-CoV-2 and Huh7 for WNV and CHIKV infections.
Quantification of viruses demonstrates antiviral efficacy.
Cell toxicity must maintain above the threshold for accurate results.

Abstract

When evaluating the antiviral effect of a compound, assessing cellular toxicity in the target cell line is an essential prerequisite (see dedicated SOP). The highest dose allowing > 90 % cell viability is the first tested dose in antiviral assay. Quantifying viruses in the supernatants of infected cell cultures enables the evaluation of the antiviral efficacy of compounds. Vero E6 cell line is used for SARS-CoV-2 infection and Huh7 cell line for WNV and CHIKV infection.

اسأل الذكاء الاصطناعي

Bookmark

View Full Paper