Introduction: Siddha polyherbal formulations possess significant therapeutic value, but their molecular characterization remains limited. This study aims to analyze the functional groups present in Rashnadi Chooranam using Fourier Transform Infrared Spectroscopy (FTIR). Methods: The formulation was prepared following standard Siddha procedures. FTIR analysis was performed in the mid-infrared region (4000–500 cm⁻¹), and spectral peaks were interpreted to identify functional groups. Results: The spectrum showed O–H and N–H stretching (phenols and amines), aliphatic C–H groups (lipids and terpenoids), C=C and amide bands (flavonoids and proteins), and C–O/C–O–C stretching (glycosides and carbohydrates). The fingerprint region indicated complex plant-derived compounds. Conclusion: The study confirms the presence of diverse bioactive constituents in Rashnadi Chooranam and supports the use of FTIR as a reliable method for standardization and quality evaluation of Siddha formulations.
Supriya K. S.1*, Noorul Hasina R.2, Uma Kalyani S.3 (Mon,) studied this question.