Background and Aim: Studies have confirmed the significance of microRNAs (miRNAs) in various biological processes. Various miRNAs have been studied in diabetes mellitus, among them miR-146a and miR-196a2 play an important role in the development of this disease. This research aims to examine the frequency of rs2910164, rs57095329 polymorphisms in miR-146a and rs11614913 in miR-196a2, T2DM patient comparing to control group. Methods: One hundred patients with T2DM and 100 healthy individuals as normal control group were included in this study. Polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) was exploited for genotyping of rs2910164C>G and rs11614913C>T and amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) was used for rs57095329A>G. Genotypes frequencies were evaluated with chi square test. Results: We found that the GG genotype rs2910164 is significantly associated with T2DM susceptibility (OR = 12.1, 95% CI = 1.36-7.9, P = 0.003). Another investigation showed a significant association between the TT genotype of rs11614913 polymorphism and T2DM susceptibility (OR = 4.36, 95% CI = 1.13-5.84, P = 0.006). The GG genotype of rs57095329 showed a significant difference between T2DM and controls (OR = 8.37, 95% CI = 3.07 - 22.82, P G, rs11614913C>T, and rs57095329A>G, were associated with an increased risk of type 2 diabetes mellitus (T2DM) in the Iranian population. These findings suggest that these variants may be new risk factors in the development of T2DM.
Shirazi et al. (Wed,) studied this question.