Regeneration-on-a-chip: a planarian microfluidic device enabling automated cultivation, individual tracking and in vivo imaging for regeneration study

Key Points

• The research aims to develop a microfluidic device for studying planarian regeneration and tracking individual responses.
• Developed a microfluidic system for automated culture of freshwater planarians.
• Enabled individual tracking and real-time in vivo imaging to observe regenerative processes.
• Evaluated the system's efficiency in various experimental setups.
• Successfully cultivated planarians with a high survival rate over extended periods.
• Achieved accurate tracking of individual planarian regeneration responses.
• Real-time in vivo imaging provided insights into stem cell behavior during regeneration.

Abstract